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Determination of histology score (H-score)

YH Yu-Hsiang Huang
PC Pei-Yi Chu
JC Ji-Lin Chen
CH Chun-Teng Huang
CH Chi-Cheng Huang
YT Yi‐Fang Tsai
YW Yu-Ling Wang
PL Pei-Ju Lien
LT Ling-Ming Tseng
CL Chun-Yu Liu
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H-score was calculated by using a semi-quantitative assessment of the percentage of positive-stained carcinoma cells (from 0 to 100) and the staining intensity (graded as 0, non-staining; 1, weak; 2, moderate and 3, strong). The range of H-score was from 0 to 300. Board certified pathologists specialized in breast cancer evaluated and scored the H-score independently without given the clinical information of tumor specimen, as previously described53. Expression level of GPNMB was categorized into high and low subgroup according to the cut-off value determined by ROC curve analysis.

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