2.10. IC50 calculation and statistical analysis

The IC₅₀ calculation and statistical analysis were performed on Graph pad prism 6.0. The Kruskall Wallis test and the Dunn test were applied to make multiple comparisons between the treatments and the negative control. Also, the Mann Whitney test to compare given concentrations versus the control. The Cytotoxicity assay increases concentrations to obtain the Median toxicity dose (TD₅₀), as the concentration increases, the viability of HFF cells decreases. The cytotoxicity test allows us to approximate the mean TD₅₀ toxicity, a measure of cytotoxicity, and helps to find the therapeutic index calculated by TD₅₀/IC₅₀. We use the data obtained with the strain ME49 to have a concentration gradient of the effect of molecules so that calculate the IC₅₀ after the transformation and normalization of data (Supplementary data).