2.5.5. Western Blot Analysis of Stomach Tissue

Another part of the stomach tissue stored at -80°C is added with protease inhibitor and homogenized with cold normal saline and centrifuged at 12000 rpm at 4°C for 10 min, and the supernatant was taken for standby. After the preparation of polyacrylamide gel, gel perfusion, sample addition, electrophoresis, transfer membrane, chemical reaction, and immobilization, the experimental images were obtained. Finally, the alpha software processing system was used to process the film image and analyzed the optical density of the target band. The contents of COX-2, NF-κB p65, p-NF-κB p65, IKBα, and p-IKBα were all determined by this way.