RNA extraction, qRT–PCR, and mRNA half‐life assays

Total RNA isolation, cDNA synthesis, and qRT–PCR analysis were performed as previously described by us (Yang et al, 2015) with some modification. Expression of ADARB1, CELF2, QKI, ZFP36, QKI‐5/6/7, TGFβR1, E‐cadherin, N‐cadherin, PAI‐1, Slug, Snail, KLF6, etc., was normalized to β‐actin. Relative mRNA levels were calculated using the ΔΔCt method. For mRNA half‐life assay, 10 μg/ml actinomycin D (Sigma‐Aldrich, St. Louis, MO, USA) was added to A549 and H1299 cells. Total RNAs were extracted at the indicated time points for qRT–PCR assay. Primers used for qRT–PCR assay are listed in Appendix Table S5. Each RT–qPCR analysis was carried out three times.