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MTT assay was performed to test cell proliferation. Transfected PC cells were seeded into 96-well plates and then incubated for 12 hours. 20 μl of MTT (Sigma-Aldrich, USA) was added into 96-well plates and incubated for 4 hours in a humidified 37˚C incubator with 5% CO2 . Then, medium was removed and 150 μl dimethyl sulfoxide (DMSO, Sigma-Aldrich, USA) was added into 96-well plates. Finally, following shaking at 25˚C for 15 minutes, the optical density was measured at 490 nm by microplate reader (Bio-Rad Laboratories Inc., USA).
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