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4.10. Bisulfite genomic sequence PCR (BSP)

LZ Lijie Zhou
CZ Cai Zhang
XY Xiong Yang
LL Lilong Liu
JH Junyi Hu
YH Yaxin Hou
HT Hong Tao
HS Haruhiko Sugimura
ZC Zhaohui Chen
LW Liang Wang
KC Ke Chen
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BSP was performed by Servicebio (Wuhan) according to the methods described previously (81). The UCSC Genome Browser (Human assembly hg38 from December, 2013) was used to predict the human CES1 promoter‐related CpG islands (CGIs); the primers for BSP were designed by using MethPrimer online software (http://www.urogene.org/methprimer/). Treated genomic DNA samples with sodium bisulfite, and PCR was performed. Bisulfite sequencing analysis was carried out using an ABI3730XL sequencing system.

Copyright and License information:  ©2021 The Authors. published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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