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2.7. Cell Counting Kit-8 (CCK-8) Assay
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Evaluation of cell survival rates was carried out using the CCK-8 assay (KeyGen Biotechnology Co., Ltd., Nanjing, Jiangsu, China). Seeding of ~104 cells into 96-well plates was conducted. After 24 h of culture, different doses of TAA and LR12 (10 ng/ml) or supernatant from macrophages treated with TAA+LR12 were added separately. Incubation of each well was undertaken with the assistance of 10 μg CCK-8 in the dark (2 h). We also could measure absorbance (490 nm) via a multifunction microplate reader (BioTek, Montpellier, VT, USA).
Copyright and License information: ©2021 Yongjuan Wang et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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