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Caco-2 cells were incubated in medium containing 100 μM bile acids for 24 h. Cells were then treated with 10 μL of 2.5 mg/mL (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) MTT reagent and cultured for 2 h according to manufactures protocol. The assay was normalized to 1% DMSO vehicle control and absorbance of each well was read on a VERSAmax Microplate Reader (Molecular Devices, Sunnyvale, CA, USA) at a wavelength of 570 nm.
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This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
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