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Ethanol fixation

ND Nikita S. Divekar
AD Amanda C. Davis-Roca
LZ Liangyu Zhang
AD Abby F. Dernburg
SW Sarah M. Wignall
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Worms were either allowed to grow on regular NGM plates or transferred onto auxin plates overnight. Then about 10 worms were picked into a 5μl M9 drop on a microscope slide and the M9 was wicked away using Whatman paper. Then 5μl of 100% ethanol was added to the slide and allowed to evaporate, this process was repeated two more times. The worms were then mounted using a solution of 50% diluted Hoechst (1:1000 in M9) and 50% mounting media (0.5% p-phenylenediamine in 90% glycerol, 20mM Tris, pH 8.8). The slides were imaged within 5 hours of preparation.

Copyright and License information:  ©2021 Divekar et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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