Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Ask a
question
Favorite
Fluoro-Jade C staining was performed according to the manufacturer’s protocol (Merck). Deparaffinized tissue sections were incubated for 10 min in 0.06% potassium permanganate solution (Merck). After washing with distilled water, samples were incubated in 0.0001% Fluoro-Jade solution in distilled water with 0.1% acetic acid (Merck) for 20 min. Sections were washed with distilled water, cleared with xylene, and mounted with D.P.X. Ambient light was blocked as much as possible during the entire procedure.
Copyright and License information: ©2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).
This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
