Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Cell viability assay

QZ Qingxia Zhao
AP Amanda B. Parris
EH Erin W. Howard
MZ Ming Zhao
ZM Zhikun Ma
ZG Zhiying Guo
YX Ying Xing
XY Xiaohe Yang
request Request a Protocol
ask Ask a question
Favorite

Cells were seeded (1 × 103 cells/well) in 96-well plates for 24 hours. The next day, indicated concentrations of FGFR inhibitors were added to fresh medium and the cells were incubated for 5 days. After 5 days, cell viability was assessed using a CellTiter 96 AQueous Non-Radioactive Cell Proliferation kit (Promega; Madison, WI) according to manufacturer’s instructions. Briefly, MTS reagent was added to the cells for 2 hours, then absorbance at 490 nm was measured using a SynergyMx microplate reader (BioTek; Winooski, VT). The viable cells are expressed as the cell viability fraction of FGFR inhibitor-treated cells as compared to the untreated control cells.

Copyright and License information: The Author(s) ©2017
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A