2.1. Materials and Reagents

DMEM/F12, cat. C470п (PanEco, Moscow, Russia); Fetal Bovine Serum, qualified, One Shot format, Brazil, Gibco, cat. A3160802 (Thermo Fisher Scientific, Waltham, MA, USA); GlutaMAX Supplement, Gibco, cat. 35050061 (Thermo Fisher Scientific, Waltham, MA, USA); ITS, cat. Φ065 (PanEco, Moscow, Russia); EGF cat. CB-1101001 (PanEco, Moscow, Russia); FGF-2, cat. CB-1102021 (PanEco, Moscow, Russia); Dispase II, Sigma-Aldrich, cat. D4693 (Merck Life Science, Amsterdam, The Netherlands); Antibiotic-Antimycotic (100X), Gibco, cat. 15240062 (Thermo Fisher Scientific, Waltham, MA, USA).

Purified anti-Nestin Antibody, cat. 656802 (Biolegend, San Diego, CA, USA); Anti-GFAP Antibody, cat. 840001 (Biolegend, San Diego, CA, USA); Anti-TAGLN antibody, Sigma-Aldrich, cat. HPA019467 (Merck Life Science, Amsterdam, the Netherlands); Anti-Actin, α-Smooth Muscle antibody, Mouse monoclonal, Sigma-Aldrich, cat. A5228 (Merck Life Science, Amsterdam, the Netherlands); Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Texas Red, Invitrogen, cat. T-2767 (Thermo Fisher Scientific, Waltham, MA, USA); Alexa Fluor^® 488 Goat anti-mouse IgG (minimal x-reactivity) Antibody, cat. 405319 (Biolegend, San Diego, CA, USA).

RNeasy column kits, cat. 74104 (Qiagen, Hilden, Germany); RNAlater RNA Stabilization Reagent, cat. 76104 (Qiagen, Hilden, Germany); DNase I, cat. M0303 (NEB, Ispwich, MA, USA); CleanRNA Standard kit, cat. BC033 (Evrogen, Moscow, Russia); NEBNext Ultra II Directional RNA Library Prep, cat. E7765 (NEB, Ispwich, MA, USA); NEBNext Poly(A) mRNA Magnetic Isolation Module, cat. E7490 (NEB, Ispwich, MA, USA); NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1), cat. E7600 (NEB, Ispwich, MA, USA).