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Generation of AAV Vectors Expressing shRNAs.

RM Roberta Marongiu
MA Margarita Arango-Lievano
VF Veronica Francardo
PM Peter Morgenstern
XZ Xiaoqun Zhang
MC M. Angela Cenci
PS Per Svenningsson
PG Paul Greengard
MK Michael G. Kaplitt
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To silence p11 expression, we generated an shRNA against mouse p11 shRNA (sh.p11) and a luciferase shRNA (sh.Luc) as a negative control. These were packaged into AAV vectors using a two-plasmid system in HEK 293 cells. The production and purification procedures are described in detail in SI Materials and Methods.

Copyright and License information:  ©2016-01-19
Freely available online through the PNAS open access option.

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