The wet/dry weight (W/D) ratios were calculated as an indicator of pulmonary edema (32). Briefly, the right upper lung of each mouse was removed, rinsed in saline and then weighed to determine the wet weight. The lung was then dried at 80°C in an oven for 48 h and reweighed to obtain the dry weight. The W/D ratios were then calculated by dividing the wet weight by the dry weight.
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