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CFSE T-cell proliferation assay
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CD8+ T cells were quantified by isolating T cells from the spleens of wild-type BALB/c mice and stained with 10 μM of cell proliferation dye eFluor 450 and incubated at 37 °C for 4 days. The cells were then harvested and stained for CD8+ T cells using CD8 antibody and FACS analysed for CD8+ and eFluor 450.
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