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Detection of NO production by Griess reagent
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NO production was assessed based on the accumulation of nitrite in the medium using a colorimetric reaction with Griess reagent [0.1% N-(1-naphthyl) ethylenediamine dihydrochloride, 0.1% sulfanilamide, and 2.5% H3PO4]. Culture supernatants were collected and mixed with an equal volume of Griess reagent. Absorbance was measured at 540 nm using the UV Max Kinetic Microplate Reader.
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