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Magnetic-activated cell sorting (MACS)

JY Jeong-Eun Yoo
DL Dongjin R. Lee
SP Sanghyun Park
HS Hye-Rim Shin
KL Kun Gu Lee
DK Dae-Sung Kim
MJ Mi-Young Jo
JE Jang-Hyeon Eom
MC Myung Soo Cho
DH Dong-Youn Hwang
DK Dong-Wook Kim
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To isolate cell surface marker-positive cells via MACS, expanded cells at D20 were dissociated into single cells as described above. Dissociated cells were stained with primary antibodies (Supplementary Table 3) in 1% FBS-PBS solution for 30 min at 4 °C, and labeled cells were incubated with 20 µl Microbeads (Miltenyi Biotec) per 1 × 107 total cells for 30 min. Marker-positive cells were separated from marker-negative cells using a separation column (LS column; Miltenyi Biotec) following the manufacturer’s instructions. Sorted cells were replated on Matrigel-coated culture dishes in N2B27 medium. After two days, in vitro characterization and in vivo transplantation were performed.

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