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Polyphenol oxidase (PPO)
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Polyphenol oxidase enzyme activity was assayed by the method of Taneja and Sachar [69]. The assay mixture contained 1.8 ml of assay buffer, 2 ml catechol solution as substrate and 0.2 ml enzyme extract in glass test tubes. These test tubes were incubated at 37 °C for 1 h to take place the assay reaction followed by measuring absorbance at 430 nm on a UV–Vis spectrophotometer. A separate blank was prepared for each sample, simultaneously by taking boiled enzyme extract. The enzyme activity was expressed as a change in 0.01 absorbance min−1 mg−1 protein.
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