Glutathione reductase activity (GR)

Praveen Kumar

Improve Research Reproducibility A Bio-protocol resource

Home
Protocols

Concise Method

Glutathione reductase activity (GR)

PK Praveen Kumar

This method is extracted from research article: BMC Plant Biol, Jul 2021

Soil applied glycine betaine with Arbuscular mycorrhizal fungi reduces chromium uptake and ameliorates chromium toxicity by suppressing the oxidative stress in three genetically different Sorghum (Sorghum bicolor L.) cultivars

DOI: 10.1186/s12870-021-03113-3

Request a Protocol

Ask a question

Favorite

Glutathione reductase was assayed using the procedure of Halliwell and Foyer [67]. The assay mixture (3.0 ml) contained 2.5 ml of assay buffer, 0.2 ml EDTA, 0.15 ml of 50 mM oxidized glutathione, 0.1 ml of 30 mM NADPH and 50 µl of enzyme extract. Assay reaction was initiated by adding NADPH at the end. A decrease in absorbance was recorded simultaneously, at 340 nm wavelength against a suitable blank containing boiled enzyme extract. The amount of NADPH oxidized was calculated by using an extinction coefficient (Absorbance of one molar solution) of 6.12 mM⁻¹ cm⁻¹ in the standard equation for absorbance. One unit activity of the enzyme corresponded to the amount of enzyme required in the oxidation of one nmol of NADPH min⁻¹.

The standard equation for absorbance as A = ε × Ɩ × c.

Where A is the amount of light absorbed by the sample at a given wavelength, ε is the molar extinction coefficient, Ɩ is the distance that the light travels through the solution, and c is the concentration of the absorbing species.

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

Post a Question

0 Q&A

Share your protocol with your peers.

Submit a Preprint Protocol