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Peroxidase (POD) activity

TK Tehmina Kiani
FM Farrakh Mehboob
MH Muhammad Zeeshan Hyder
ZZ Zainy Zainy
LX Liangsheng Xu
LH Lili Huang
SF Sumaira Farrakh
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POD activity was quantified in a spectrophotometer by oxidizing the 0.1 mL protein sample in 3 mL of 0.05 M phosphoric acid buffer, pH 5.5, 2 mL of 2% hydrogen peroxide (H2O2) as the oxidant, and 36 μL guaiacol as the hydrogen donor per 50 mL buffer. The oxidation of guaiacol was measured at 470 nm at 25 °C and was expressed in U g−1 FW min−159.

Enzyme activity was defined as “an increase in absorbance of 1 unit enzyme at 470 nm min−1 at 25 °C”. The specific activity of POD was expressed in U mg protein−1.

The enzyme activity is calculated from the following formula:

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