Macrophage and hucMSCs co-culture

ZT Zan Tang
JG Junxiao Gao
JW Jie Wu
GZ Guifang Zeng
YL Yan Liao
ZS Zhenkun Song
XL Xiao Liang
JH Junyuan Hu
YH Yong Hu
ML Muyun Liu
NL Nan Li
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hucMSCs (1 × 104/cm2) were seeded in a 24-well plate 24 h before co-culture. Macrophages were sorted from the mice lung 1 day after bleomycin treatment and co-cultured with hucMSCs (10:1). The hucMSCs and macrophages were cultured in RPMI-1640, 10% FBS, and 1% Penicillin/Streptomycin. mRNA was extracted after co-culture for 72 h.

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