EdU proliferation assay

ZT Zan Tang
JG Junxiao Gao
JW Jie Wu
GZ Guifang Zeng
YL Yan Liao
ZS Zhenkun Song
XL Xiao Liang
JH Junyuan Hu
YH Yong Hu
ML Muyun Liu
NL Nan Li
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For assessing cell proliferation in vivo, EdU (Thermo Fisher Scientific, Catalog#E10415) was administered to mice by intraperitoneal injection at 50 mg of EdU per kg of the mouse (weighed 24 h prior to sacrifice). EdU incorporation was detected using the Click-iT EdU Alexa Fluro 488 flow cytometry assay kit (Invitrogen, catalog#C10632).

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