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Vero-E6 cells (100,000 cells per well) were seeded in 24-well plate. Cells were infected with SARS-CoV-2 (MOI, 0.005 pfu/cell) for 4 h at 37 °C. The monolayers were then washed and overlaid with medium supplemented with 5% FBS containing serial dilutions of compound, alternatively the compound was added 24 hpi. Supernatants were harvested 48 hpi and titrated on Vero-E6 cells.
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