Tumor Growth Study

NCr homozygous athymic (nude) mice (eight weeks-old) were purchased from Charles River Laboratories. An aliquot of 2×10⁶ MDA-MB-231 cells were suspended in 200 mL of PBS and injected subdermally in the right thigh of each animals. When xenograft tumors reached ~200–230 mm³ in volume, animals were regrouped into two treatment groups (n=6): Vehicle control and CRL1101 alone. After tumor cell engraftment when tumor size is palpable, on day 9, mice were treated with CRL1101 (25mg/kg) intraperitoneally every other day. Animals were maintained in accordance with guidelines of the Institutional Animal Care and Use Committee (IACUC) of Cedars-Sinai Medical Center. Tumor growth was monitored three times weekly for four weeks. Tumor volume was calculated by the formula: π/6 x (larger diameter) x (smaller diameter)². Mice body weight during the treatment was measured every 2 days.