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Measurement of Protein Kinase B Phosphorylation by Enzyme-Linked Immunosorbent Assay
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Akt serine 473 phosphorylation was measured using Pathscan phospho-Akt1 sandwich ELISA kit, according to the manufacturer’s instructions (Cell Signaling Technology). Samples were prepared from cells treated with 10-nM T3 for 24 hours and then 1 hour incubation in the presence or absence of 1- or 10-µM LY294002. A total of 100 μL of samples containing equal amount of protein were applied to each well.
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