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4.5.5. Determination of Antioxidant Activity by DPPH Assay
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An amount of 0.5 mL of BRB was added to 0.5 mL of absolute ethanol at different concentrations and then mixed with 0.6 mL of 100 mM DPPH for 30 min under light protection. The absorbance was read at 517 nm on a spectrophotometer. The DPPH radical scavenging activity was calculated. The antioxidant activity was expressed as gallic acid equivalent (GAE).
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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