2.5. LPS Cell Lines and Patient-Derived 18DDLPS

De-differentiated human liposarcoma cell lines LPS246 and LPS863 were provided by Dr. Dina Lev (MD Anderson Cancer Center, Houston, TX, USA). Well-differentiated human liposarcoma cell lines 93T449 and 94T778 were purchased from American Type Culture Collection (Manassas, VA, USA; #CRL-3043 and #CRL-3044, respectively). The 18DD primary cells were established by the Sarcoma Research Center at SMC. The tumor material was excised aseptically from a 74-year-old female patient and then processed as follows: enzymatic dissociation to single cells using sequential incubation in 300 U/mL collagenase (Sigma-Aldrich, St. Louis, MO, USA) at 37 °C and 100 U/mL hyaluronidase (Sigma-Aldrich) for 2.5 h, 0.25% trypsin/EDTA (STEMCELL Technologies, Vancouver, BC, USA) for 4 min, then 5 U/mL dispase (STEMCELL Technologies) plus DNAse (100 μg/mL, Sigma-Aldrich) for 4 min, before passing through a 40 µm filter. LPS246, LPS 863, and 18DD were cultured in DMEM, and 93T449 and 94T778 were cultured in RPMI-1640 supplemented with 10% heat-inactivated fetal bovine serum and 100U/mL of the antibiotics at 37 °C in a humidified incubator with 5% CO₂.