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The gel percent of the hydrogels was determined gravimetrically. The different lyophilized hydrogels were previously weighted, and subsequently immersed in 10 mL of PBS for 96 h at room temperature under slight shaking, and then freeze-dried. The gel percent was calculated by the following equation:
where wi and ww are the weights (g) of the hydrogel before and after washing to extract the soluble parts, respectively. All the experiments were performed in triplicate.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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