GEPIA2

GEPIA2 (gene expression profiling interactive analysis, version 2; http://gepia2.cancer-pku.cn/#analysis) is an interactive web server for analyzing RNA expression data from tumors and normal samples from TCGA and genotype-tissue expression (GTEx) projects [38]. In this study, the “Expression Analysis-Box Plots” module of GEPIA2 was used to obtain box plots of PXN expression between tumor and normal tissues from the GTEx database. We set the P-value cutoff = 0.01, log₂FC (fold change) cutoff = 1, and “Match TCGA normal and GTEx data”. The “Survival Map” module of GEPIA2 was used to obtain the overall survival (OS) and disease-free survival (DFS) significance map data related to PXN across all tumors in TCGA. We set cutoff-high (50%) and cutoff-low (50%) values to split the high-expression and low-expression cohorts. The survival data were visualized with hazard ratio, 95% confidence intervals, and log-rank P values. The “Pathological Stage Plot” module of GEPIA2 was used to obtain PXN expression in different pathological stages of different tumors in TCGA. The “Similar Gene Detection” module of GEPIA2 was used to identify the top 100 PXN-correlated targeting genes from TCGA.