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Quantification of cellular viability and proliferation.

CD Constance Duchesne
NF Nadira Frescaline
OB Océane Blaise
JL Jean-Jacques Lataillade
SB Sébastien Banzet
OD Olivier Dussurget
AR Antoine Rousseau
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Cellular viability was determined using the stable tetrazolium salt WST-1 reagent (Sigma-Aldrich, St. Louis, MO), which is cleaved to soluble formazan by cellular mitochondrial dehydrogenases. RAW 264.7 cells were treated with CAP for 30 s, and the WST-1 assay was conducted according to the manufacturer’s instructions at 24 h and 48 h posttreatment. Absorbance was measured using a microplate reader (Labsystems, Finland) at 450 nm.

Copyright and License information:  ©2021 Duchesne et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.

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