Comparative proteomics was conducted by collecting Thraustochytrium sp. cells grown with or without 5 g/liter of NaCl in the EM. About 10 OD of cells were collected at the time point of 60 h by centrifuging at 10,000 × g for 2 min. The proteomic sample preparation and mass spectrometry analysis in a Thermo LTQ Orbitrap XL mass spectrometer were conducted following the same protocol as that in our previous study (48). The raw data collected from tandem mass spectrometry (MS/MS) analysis were searched and analyzed using programs integrated into the PatternLab for Proteomics (49). Peptide information was obtained by searching against the database obtained from translating our transcriptomic data (data not published).
This is a work of the U.S. Government and is not subject to copyright protection in the United States. Foreign copyrights may apply.This is a work of the U.S. Government and is not subject to copyright protection in the United States. Foreign copyrights may apply.This is a work of the U.S. Government and is not subject to copyright protection in the United States. Foreign copyrights may apply.This is a work of the U.S. Government and is not subject to copyright protection in the United States. Foreign copyrights may apply.This is a work of the U.S. Government and is not subject to copyright protection in the United States. Foreign copyrights may apply.This is a work of the U.S. Government and is not subject to copyright protection in the United States. Foreign copyrights may apply.This is a work of the U.S. Government and is not subject to copyright protection in the United States. Foreign copyrights may apply.This is a work of the U.S. Government and is not subject to copyright protection in the United States. Foreign copyrights may apply.This is a work of the U.S. Government and is not subject to copyright protection in the United States. Foreign copyrights may apply.This is a work of the U.S. Government and is not subject to copyright protection in the United States. Foreign copyrights may apply.
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