Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Actinomycin D Transcription Inhibition Assay
Request a Protocol
Ask a
question
Favorite
Approximately, 2.0 × 105 Huh7 cells were seeded in 12-well plates and cultured until complete adherence. After that, actinomycin D (Abcam, United States) was added to the culture media at a final concentration of 2 μg/ml. Then, the cells in wells were collected for RNA extraction at 0, 3, 6, 12, and 24 h, respectively. The RNA samples were subjected to reverse transcription and RT-qPCR systems.
Copyright and License information: ©2021 Liu, Tian, Chen, Shen, Xia, Nan, Yan, Wang, Shi, Shen, Yu and Cai.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
