Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
2.11. Measurement of alanine aminotransferase (ALT) and aspartate transaminase (AST) in serum
Request a Protocol
Ask a
question
Favorite
Fresh blood (5 mL) was collected from mice and centrifuged at 1000 g to collect the serum. The ALT and AST levels released from liver tissue were measured using an AU5800 series automatic biochemical analysis system (Beckman Coulter Laboratory Systems Co Ltd) and ALT/AST‐specific detection kits (Beckman Coulter Laboratory Systems). The result was used to determine the injury on hepatocytes.
Copyright and License information: ©2021 The Authors. published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
