2.4. Transfection of siRNA

Human PDAC cells were transfected with control siRNA (siControl, Thermo Fisher Scientific), HSP47‐A siRNA (siHSP47‐A, sense: 5′‐CUACGACGACGAGAAGGAATT‐3′; antisense: 5′‐UUCCUUCUCGUCGUCGUAGTA‐3′, Thermo Fisher Scientific) and HSP47‐B siRNA (siHSP47‐B, sense: 5′‐AGCCCUCUUCUGACACUAATT‐3′; antisense: 5′‐UUAGUGUCAGAAGAGGGCUGG‐3′, Thermo Fisher Scientific) using Lipofectamine RNAiMAX (Thermo Fisher Scientific) and were cultured in Opti‐MEM reduced medium (Thermo Fisher Scientific) for 5 h at 37°C in air. At 5 h after transfection with siRNA, the cells were cultured in RPMI1640 medium (for PANC1 cells) or DMEM (for MIA‐PaCa2 cells and Suit2 cells) supplemented with 10% FBS.