CCA were performed on citrated plasma (Vacuette® Greiner, Kremsmünster, Austria; trisodium citrate 3.8% 9:1 v/v). We assessed prothrombin time (PT; Owren, reference range: 70–125%), activated partial thromboplastin time (aPTT; reference range: 27–41 s), antithrombin (AT; reference range: 80–120%), fibrinogen (Clauss method; reference range: 200–400 mg dl−1), and antiXa levels (STA®-Liquid Anti-Xa, REF 00311 and REF 00322, reference range: <0.1 IU ml−1) via the STA R Max 2® coagulometer (Diagnostica Stago SAS, Asnières-sur-Seine, France). Platelet count (reference range: 150–350 G l−1) was determined from an EDTA tube (Vacuette® Greiner, Kremsmünster, Austria) via the Sysmex XE- 2100 cell counter (Sysmex, Kobe, Japan).
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