Patients

Between December 2002 and November 2008, 657 patients between the ages of 17 and 60 years with de novo untreated AML were enrolled on ECOG-ACRIN Cancer Research Group study E1900 (#{"type":"clinical-trial","attrs":{"text":"NCT00049517","term_id":"NCT00049517"}}NCT00049517) as detailed in Fernandez et al.¹⁷ Cytogenetic risk assignment was according to Slovak et al.²⁰ Patients were classified as indeterminate risk if they had unknown cytogenetic risk as per Slovak et al or lacked sufficient data for classification. Testing for presence of an FMS-like tyrosine kinase internal tandem duplication (FLT3-ITD) and mixed-lineage leukemia partial tandem duplication (MLL-PTD) was conducted centrally as previously described.^17,21-23 Sequencing of NPM1, DNMT3A, IDH1/2, NRAS, CEBPA, TET2, WT1, KIT, RUNX1, ASXL1, PHF6, KRAS, PTEN, and TP53 was later conducted on banked samples as described by Patel et al.¹⁸ FLT3-ITD allelic ratio was not available. The median follow-up time from the patients still alive and included in the current analysis, calculated from the time of randomization for induction therapy, was 80.1 months (range, 0.8-120.4 months). In total, 25 (4%) patients were lost to follow-up.