4.3. SiRNA Transfection of A549 and MRC5 Cells

Small interfering RNA (siRNA), including the scrambled siRNA control, was purchased from Ambion (Huntingdon, Cambridge, UK; catalogue no. 4390843). MUC16 gene-targeted siRNA (identification no. IDS41226, catalogue no. 4392420) was designed by Ambion. Cells were transfected with siRNA (50 nM) in serum and antibiotic-free medium. After 6 h, the medium was aspirated and replaced with medium containing serum for a further 42 h before cell stimulation. The transfection reagent used was lipofectamine-2000 (Invitrogen, Paisley, UK; catalogue no. 11668-027) at a final concentration of 2 µg/mL.