To predict the miRNA-targeted mRNAs, the online miRWalk v3.0 tool (http://mirwalk.umm.uni-heidelberg.de/ accessed on 17 June 2021) was used [54]. After obtaining a list of validated mRNAs genes relative to the seven miRNAs, we scanned these genes and analyzed them for the cellular, molecular, and biological process of GO analysis and Kyoto encyclopedia of genes and genomes (KEGG) pathway.
All sequence data described here are available in the online Zenodo repository https://doi.org/10.5281/zenodo.4657056 (accessed on 17 June 2021).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.