Peptide preparation

LP L. Palanikumar
LK Laura Karpauskaite
MA Mohamed Al-Sayegh
IC Ibrahim Chehade
MA Maheen Alam
SH Sarah Hassan
DM Debabrata Maity
LA Liaqat Ali
MK Mona Kalmouni
YH Yamanappa Hunashal
JA Jemil Ahmed
TH Tatiana Houhou
SK Shake Karapetyan
ZF Zackary Falls
RS Ram Samudrala
RP Renu Pasricha
GE Gennaro Esposito
AA Ahmed J. Afzal
AH Andrew D. Hamilton
SK Sunil Kumar
MM Mazin Magzoub
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Peptides (pWT, pR248W and ReACp53; sequences shown in Fig. 1c) were synthesized by Selleck Chemicals (Houston, TX) using standard Fmoc methods. The peptides were purified inhouse by reverse-phase high-performance liquid chromatography (Waters 2535 QGM HPLC), and purity was subsequently verified using mass spectrometry (Agilent 6538 QToF LC/MS). After purification, peptides were aliquoted into 0.5 mL Protein LoBind tubes (Eppendorf), lyophilized and stored at −80 °C until needed. For each experiment, fresh peptide stock solutions were prepared in DMSO and filtered using 0.22 μm Ultrafree-MC spin filters. pR248W concentration was determined by absorbance measurements at 280 nm (ε = 5690 cm–1 M–1 for tryptophan) on a Lambda 25 UV/Vis Spectrophotometer (PerkinElmer, Waltham, MA) using quartz cuvettes (1 cm path-length).

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