Peptides (pWT, pR248W and ReACp53; sequences shown in Fig. 1c) were synthesized by Selleck Chemicals (Houston, TX) using standard Fmoc methods. The peptides were purified inhouse by reverse-phase high-performance liquid chromatography (Waters 2535 QGM HPLC), and purity was subsequently verified using mass spectrometry (Agilent 6538 QToF LC/MS). After purification, peptides were aliquoted into 0.5 mL Protein LoBind tubes (Eppendorf), lyophilized and stored at −80 °C until needed. For each experiment, fresh peptide stock solutions were prepared in DMSO and filtered using 0.22 μm Ultrafree-MC spin filters. pR248W concentration was determined by absorbance measurements at 280 nm (ε = 5690 cm–1 M–1 for tryptophan) on a Lambda 25 UV/Vis Spectrophotometer (PerkinElmer, Waltham, MA) using quartz cuvettes (1 cm path-length).
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