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BODIPY® FL maleimide labeling of purified ABCB4

TK Tim Kroll
SS Sander H.J. Smits
LS Lutz Schmitt
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ABCB4 was specifically inhibited by labeling the unique cystine of the Walker A motif as described for ABCB1 (58) and ABCB4 (54). A sample of purified ABCB4 was incubated with 10-fold molar excess of BODIPY® FL maleimide (BODIPY® FL N-(2- aminoethyl)maleimide, Molecular Probes) at 22°C for 20 min. The reaction was quenched after 20 min by addition of a 20-fold molar excess of dithiothreitol (DTT) at 22°C for 10 min. Qualitative labeling efficiency was analyzed by visualizing a sample after SDS-PAGE by UV excitation.

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This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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