2.2. Preparation of Peptide Solutions

Peptide solutions were prepared by dissolving 10 mg of each lyophilized peptide in 1.0 mL of water. These suspensions were sonicated for 30 min at room temperature; after centrifugation (5 min at 13,000 rpm), the concentration was spectroscopically determined on UV–Vis Thermo Fisher Scientific Inc. (Wilmington, DE, USA) NanoDrop 2000 c spectrophotometer equipped with a 1.0 cm quartz cuvette (Hellma). The quantification of the concentration was assessed using the molar absorptivity (ε) of 7800 M⁻¹ cm⁻¹ for Fmoc group at λ = 301 nm.