4.16. Novel Object Recognition Test (NOR)

The object discrimination test, also known as the spontaneous object recognition test or the new object recognition test (NOR), is used to assess the ability to recognize a new object in the environment, which is the most popular [79]. The working procedure was like that described in previous studies by our research group [29,42]. In short, it is used to assess the ability to recognize a new object in the environment. This is one of the most popular tests used to assess short- and long-term memory [79]. This behavioral test was performed in a glass aquarium of ~20 L with dimensions of 30 × 30 × 30 cm³ (length × width × height). Before the experiment began, the animals underwent training for 4 days. After training, the animals underwent a retention interval of one hour while they were given treatment. To avoid the influence of thigmotaxis, the distances between objects and walls were kept the same.