4.11. Intracranial Tumor Model

Intracranial injection was performed as previously described by us [65]. HCC1806-luciferase expressing cells were used for this purpose and these cells were injected in 4–6 weeks old female athymic nude mice. Approximately 0.1 × 10⁶ cells in a cell suspension of 5 µL were injected in the brain of each mouse using stereotaxic apparatus at a flow rate of 1 µL/min. After dividing the mice into two groups (n = 9), 50 mg/kg ATQ was administered orally every day in treated group. The tumor growth in mice brain was monitored through a non-invasive imaging technique. At Day 35, mice were humanely sacrificed by CO₂ overdose, and brains from control and treated group was dissected out and imaged for luminescence using IVIS imaging.