Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
2.4.1. Primary Culture of Aspergillus niger
Request a Protocol
Ask a
question
Favorite
After cultivation for 5–7 days of the isolated fungus, a small amount of inoculum was collected and mixed with 6–8 mL sterilized water. Later, a small amount of spore suspension was inoculated in 50 mL of submerged culture medium in conical flasks and then cultivated in a rotary shaker incubator at 28 ± 2 °C at 150 rpm for 5–7 days.
Copyright and License information: ©2021 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
