Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Expression of SPM Receptor Genes in Gingival Tissue Samples via qRT-PCR

CL Chun-Teh Lee
RL Ruoxing Li
LZ Lisha Zhu
GT Gena D. Tribble
WZ W. Jim Zheng
BF Brittney Ferguson
KM Krishna Rao Maddipati
NA Nikola Angelov
TD Thomas E. Van Dyke
request Request a Protocol
ask Ask a question
Favorite

Expression of SPM corresponding receptor genes was analyzed with qRT-PCR, utilizing the TaqMan Gene Expression Assays Protocol (Applied Biosystems, Foster City, CA, USA). Details of the experiment were previously described (21). The TaqMan Assay primers included GAPDH (assay ID: Hs99999905_m1, Applied Biosystems) as the housekeeping gene compared to ALX (FPR2) (assay ID: Hs00265954_m1, Applied Biosystems), BLT1 (LTB4R) (assay ID: Hs00175124_m1, Applied Biosystems), ChemR23 (ERV1) (assay ID: Hs01386063_m1, Applied Biosystems), GPR18 (DRV2) (assay ID: Hs00245542_m1, Applied Biosystems), GPR32 (DRV1) (assay ID: Hs00265986_s1, Applied Biosystems), GPR37 (assay ID: Hs0017374_m1, Applied Biosystems),and LGR6 (assay ID: Hs00663887_m1, Applied Biosystems), which were labeled with FAM dye. The calculated cycle threshold (Ct) values from each sample were obtained and relative expression (2-ΔΔCt) was calculated to determine expression of specific SPM receptor genes between groups.

Copyright and License information:  ©2021 Lee, Li, Zhu, Tribble, Zheng, Ferguson, Maddipati, Angelov and Van Dyke
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A