Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Analysis of Transition/Transversion Ratios

MB Martin Braud
DM David A. Magee
SP Stephen D. E. Park
TS Tad S. Sonstegard
SW Sinead M. Waters
DM David E. MacHugh
CS Charles Spillane
request Request a Protocol
ask Ask a question
Favorite

To determine the transition/transversion ratio (ti/tv) in the 3′ UTR miRNA binding site SNPs, the fixed SNPs identified in the miRNA binding sites were sorted into two mutational classes based on the variants observed: a transition class (A↔G, C↔T) and a transversion class (A↔C, A↔T, C↔G, G↔T). Using a custom python script, the number of variants were summed for each group and the ti/tv ratio was calculated.

Copyright and License information:  ©2017 Braud, Magee, Park, Sonstegard, Waters, MacHugh and Spillane.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A