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Expression and purification of scFv16
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scFv16 was expressed and purified as previously described35. In brief, the 6×histidine-tagged scFv16 was expressed in secreted form in Trichoplusia ni Hi5 insect cells for 48 h using the Bac-to-Bac system. The expressed scFv16 was purified using a Ni-NTA resin. The C-terminal 6×His tag of the Ni-NTA eluent was cleaved by 3C protease and further purified by gel filtration chromatography using a Superdex 200 column. Finally, the purified scFv16 was concentrated and stored at −80 °C until further use.
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