2.19. RNAscope in situ hybridization assay

Human femoral heads were collected from patients with femoral neck fractures. An RNAscope Detection Kit from Advanced Cell Diagnostics (ACD) was used for RNAscope detection. The specimens were fixed with 10% neutral formalin and decalcified by Preserve Rapid Decalcification Solution (Pursuit Bio, Beijing, China, 0500‐0050‐500). Then, the bone tissues were embedded, and the slices were prepared. The HHAS1 probe for RNAscope detection was provided by ACD. The detection of HHAS1 in bone tissue was performed in accordance with the instructions under a light microscope.