OCT-embedded lymph nodes were sectioned into 7-μm-thick transverse sections and rinsed in TBST, depigmented with H2O2 (Sigma, H1009) for 15 min, boiled for 20 min in 10 mM sodium citrate (VWR, 1120051000) and blocked with TBST containing 3% BSA (Sigma A9418). Sections were incubated overnight at 4 °C in TBST containing 3% BSA with antibodies against SOX10 (Abcam, ab155279). Sections were then incubated in secondary donkey anti-mouse 647 (Abcam, ab150107) and goat anti-rabbit IgG (H&L)-Affinity Pure, DyLight® 650 Conjugate (GtxRb-003-E650NHSX) antibodies for 2 h at RT. All sections were counterstained with DAPI (0.5 μg/mL in ethanol, Sigma, D9542). Images were captured using a ZEISS Axio Imager 2 with Axiocam 506 color cameras. Image analysis was performed using ZEISS ZEN, Adobe Photoshop, and ImageJ software.
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