Rosettes of 5–8 leaves were mounted, frozen in nitrogen slush, platinum coated and fractured as previously described [30]. To accommodate better fractures without dislodging trichomes, some samples were dipped in 70% v/v ethanol to remove the wool and then air-dried prior to freezing. Cryo prepared samples were viewed using a Zeiss EVO HD SEM fitted with a backscattered electron detector and 25 kV acceleration voltage.
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